Localisation of three host-protective oncospheral antigens of Taenia ovis

A Jabbar; CT Kyngdon; CG Gauci; AK Walduck; C McCowan; MK Jones; I Beveridge; MW Lightowlers

Journal article

Localisation of three host-protective oncospheral antigens of Taenia ovis

A Jabbar, CT Kyngdon, CG Gauci, AK Walduck, C McCowan, MK Jones, I Beveridge, MW Lightowlers

International Journal for Parasitology | ELSEVIER SCI LTD | Published : 2010

DOI: 10.1016/j.ijpara.2009.10.008

Abstract

Immunohistochemistry, confocal immunofluorescence and immunogold labelling were used to determine the localisation of the host-protective antigens To16, To18 and To45W in Taenia ovis oncospheres. During maturation of the adult tapeworm the antigens were initially seen as diffuse staining in the developing oncospheres but in mature oncospheres four distinct cells stained positively for the antigens. Confocal fluorescence microscopy using different fluorophores revealed that each of the antigens co-localises within the same cells in the oncosphere. No surface localisation was seen in non-activated or recently activated parasites. Immunogold labelling of non-activated oncosphere sections viewed..

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University of Melbourne Researchers

Abdul Jabbar Author

Charles Gauci Author

Christina McCowan Author

Ian Beveridge Author

Related Projects (1)

Immunological prevention of cysticercosis and hydatid disease

Cysticercosis and hydatid disease are caused by infections with the larval stages of tapeworm parasites. These infections cause substantial ..

Grants

Awarded by National Health and Medical Research Council

Funding Acknowledgements

The expert technical assistance of the late Mrs. Josie Wilson, Mr. Paul Benham and Dr. Simon Crawford is greatly appreciated. We are also grateful to Mrs. Jacqueline Mills, Integrated Neuroscience Facility, Howard Florey Institute, Parkville for her assistance in co-localisation analysis. This work was supported by National Health and Medical Research Council (Grant 350279).